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Regulatory issues for genetically modified animals

Perry Bradbury HACKETT

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 188-203 doi: 10.15302/J-FASE-2019307

Abstract:

Precision genetics and breeding have the potential to meet the agricultural needs and goals of the world in the 21st century. These needs include increasing the efficiency of production of animals and improving their products with minimal impact on the environment. The USA is the major innovator in genomic science and the acknowledged leader in formulating policies to regulate genetic applications in medicine and agriculture. However, governments worldwide have been exceedingly reluctant to support the introduction of genetically modified (GM) animals into agriculture. Regulatory policies have stagnated due to legal guidelines that could not anticipate the needs and solutions that are evident today. This must change if we are to maintain planetary integrity. I propose a new, market-based regulatory model for GM livestock that has both a strong scientific foundation and has worked for 10000 years. The model is similar to that for information technology in which specific algorithms drive computer and cell phone applications. Genome engineers write genetic algorithms that drive the traits in biological organisms. Accordingly, GM products should be viewed in terms of their use and public benefit rather than by limitations to the genetic programing coming from a few highly vocal groups. Genetic algorithms (Genapps) of the 21st century will include not only introduction of synthetic genes, but also complete natural and synthetic biochemical pathways to produce agricultural products that are maximally efficient, healthy to humans and animals, and sustainable in an era of changing climates while avoiding environmental degradation.

Keywords： algorithms     editing     FDA     GMO     recombinant DNA     USDA    

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High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin

Frontiers of Chemical Science and Engineering 2007, Volume 1, Issue 4,   Pages 399-403 doi: 10.1007/s11705-007-0073-x

Abstract: The present work focused on the high expression of recombinant human consensus interferon- (cIFN) by

Keywords： bioactivity     pastoris     maximum     induction     optimized    

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Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 67-71 doi: 10.1007/s11684-009-0010-0

Abstract: In the present study, we constructed a recombinant adenovirus containing the gene of DMT1 without theIRE was then transfected into E1-transformed human embryonic kidney cells (HEK293 cells) , in which recombinantPCR confirmed pAdEasy1-DMT1-IRE contained gene DMT1-IRE, indicating the successful construction of recombinant

Keywords： divalent metal transporter 1     recombinant adenovirus     homologous recombination     iron    

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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract:

The mammary gland provides a novel method for producing recombinant proteins in milk of transgenicThe final vector containing the 8.5 kb mWAP 5′ promoter, 4.8 kb hLZ genomic DNA, and 8.0kb mWAP 3′ genomic DNA was microinjected into pronuclei of fertilized mouse embryos, to successfullygenerate two transgenic mouse lines that expressed recombinant human lysozyme (rhLZ) in milk.

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Recombinant protein diannexin prevents preeclampsia-like symptoms in a pregnant mouse model via reducing

Frontiers of Medicine 2022, Volume 16, Issue 6,   Pages 919-931 doi: 10.1007/s11684-021-0918-6

Abstract: This study describes a strategy to prevent PE by reducing MP release using the recombinant protein, diannexin

Keywords： preeclampsia     recombinant protein diannexin     microparticle     NLRP3 inflammasome     phosphatidylserin    

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Engineering DNA Materials for Sustainable Data Storage Using a DNA Movable-Type System Article

Zi-Yi Gong, Li-Fu Song, Guang-Sheng Pei, Yu-Fei Dong, Bing-Zhi Li, Ying-Jin Yuan

Engineering 2023, Volume 29, Issue 10,   Pages 130-136 doi: 10.1016/j.eng.2022.05.023

Abstract:

DNA molecules are green materials with great potential for high-density and long-term data storageHowever, the current data-writing process of DNA data storage via DNA synthesis suffers from high costsHere, we developed a DNA movable-type storage system that can utilize DNA fragments pre-produced by cellIn this system, these pre-generated DNA fragments, referred to herein as "DNA movable types,"the costly and environmentally hazardous process of de novo DNA synthesis.

Keywords： 合成生物学     DNA信息存储     DNA活字存储系统     经济性DNA数据存储    

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Clinical evaluation of recombinant human growth hormone injection in children with growth hormone deficiency

Ling HOU, Xiaoping LUO, Minlian DU, Huamei MA, Chunxiu GONG, Yuchuan LI, Shuixian SHEN, Zhuhui ZHAO, Li LIANG, Guanping DONG, Chaoying YAN, Hongwei DU

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 171-176 doi: 10.1007/s11684-009-0027-4

Abstract: Recombinant human growth hormone (rhGH) has been widely used in the clinical treatment of growth hormone

Keywords： recombinant human growth hormone     injection     growth hormone deficiency    

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supplementation of thyroid hormone on treatment of idiopathic short-stature children during therapy with recombinant

Wei Wang, Shuqin Jiang, Zhirui Cui, Xiangyang Luo, Lingli Shi, Heli Zheng

Frontiers of Medicine 2018, Volume 12, Issue 5,   Pages 580-585 doi: 10.1007/s11684-017-0585-9

Abstract: deficiency on growth rate should be considered when FT4 level lies in the low-normal range prior to recombinant

Keywords： therapeutic     idiopathic short-stature children     free T4     the first year     recombinant human growth hormone    

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The role of PARP1 in the DNA damage response and its application in tumor therapy

Zhifeng Wang, Fengli Wang, Tieshan Tang, Caixia Guo

Frontiers of Medicine 2012, Volume 6, Issue 2,   Pages 156-164 doi: 10.1007/s11684-012-0197-3

Abstract: ADP-ribosyl)ation of many key proteins in vivo and thus plays important roles in multiple DNAThe present review will focus on recent progress on the role of PARP1 in the DNA damage response andresistance of BRCA-deficient tumors to PARP inhibitors is also briefly discussed from the perspective of DNA

Keywords： PARP1     synthetic lethality     PARP inhibitor     DNA repair     cancer     NHEJ    

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Functional role of ATM in the cellular response to DNA damage

Ming LIU, Wenxiang HU

Frontiers of Chemical Science and Engineering 2011, Volume 5, Issue 2,   Pages 179-187 doi: 10.1007/s11705-009-0268-4

Abstract: our understanding of the mechanism of activation of ATM and its signaling pathways, explore whether DNA

Keywords： ataxia-telangiectasia mutated (ATM)     cell cycle checkpoint     DNA damage     signalling transduction    

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Generation and repair of AID-initiated DNA lesions in B lymphocytes

Zhangguo Chen,Jing H. Wang

Frontiers of Medicine 2014, Volume 8, Issue 2,   Pages 201-216 doi: 10.1007/s11684-014-0324-4

Abstract: AID induces U:G mismatch lesions in DNA that are subsequently converted into point mutations or DNA doubleThus, AID poses a threat to the genome of B cells if AID-initiated DNA lesions cannot be properly repairedregulate the specificity of AID targeting and the repair pathways responsible for processing AID-initiated DNA

Keywords： class switch recombination     somatic hypermutation     activation-induced deaminase     DNA repair     genomic instability    

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Molecular simulation of the interaction mechanism between CodY protein and DNA in

Linchen Yuan, Hao Wu, Yue Zhao, Xiaoyu Qin, Yanni Li

Frontiers of Chemical Science and Engineering 2019, Volume 13, Issue 1,   Pages 133-139 doi: 10.1007/s11705-018-1737-4

Abstract: In , the global transcriptional regulatory factor CodY can interact with the promoter DNA to regulateIn order to study the mechanism of interaction between CodY and its target DNA, molecular docking andARG218, SER219, ASN223, LYS242 and GLY243, among which SER186, ARG218 and LYS242 play a vital role in DNA

Keywords： CodY     DNA     molecular docking     molecular dynamics    

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Environmental pollution and DNA methylation: carcinogenesis, clinical significance, and practical applications

Yi Cao

Frontiers of Medicine 2015, Volume 9, Issue 3,   Pages 261-274 doi: 10.1007/s11684-015-0406-y

Abstract: Studies on DNA methylation are among the earliest and most conducted epigenetic research linked to cancerIn this review, the roles of DNA methylation in carcinogenesis and their significance in clinical medicinewere summarized, and the effects of environmental pollutants, particularly air pollutants, on DNA methylationFurthermore, prospective applications of DNA methylation to environmental pollution detection and cancer

Keywords： environmental pollution     DNA methylation     cancer     biomarker     diagnosis     therapy     prevention    

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Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 185-190 doi: 10.15302/J-FASE-2014035

Abstract: DNA damage is one of the most common threats to meiotic cells.Here, we reviewed exogenous factors which could induce DNA damage.Specially, we addressed the different effects of DNA damage on mouse oocytes and embryonic developmentComplex DNA damage, double-strand breaks, represents a more difficult repair process and involves variousUnderstanding the mechanisms involved in DNA damage responses may improve therapeutic strategies for

Keywords： DNA damage     double-strand breaks (DSBs)     oocyte     embryo    

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Single-Molecule Methods for Characterizing Different DNA Higher-Order Structures Review

Yonglin Liu, Tianyuan Bian, Yan Liu, Zhimin Li, Yufeng Pei, Jie Song

Engineering 2023, Volume 24, Issue 5,   Pages 277-292 doi: 10.1016/j.eng.2022.10.009

Abstract:

DNA is considered to be not only a carrier of the genetic information of life but also a highly programmableDifferent DNA structures are related to their biological and chemical functions.However, the bulk assay ignores the heterogeneity of DNA structures in solution.(ss/dsDNA), DNA higher-order structures, and DNA nanostructures.methods to understand the biophysical properties of DNA and other bio-matter and soft matter.

Keywords： Single-molecule methods     DNA structure     Mechanical properties     Conformational transitions    

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Title Author Date Type Operation

Regulatory issues for genetically modified animals

Perry Bradbury HACKETT

Journal Article

High-expression of recombinant human concensus interferon-α by Pichia pastoris

HAO Yuyou, SHI Qiqi, HE Yun, ZHUANG Yingping, WANG Yonghong, ZHANG Siliang, CHU Ju, LIU Zhimin

Journal Article

Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

Journal Article

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Recombinant protein diannexin prevents preeclampsia-like symptoms in a pregnant mouse model via reducing

Journal Article

Engineering DNA Materials for Sustainable Data Storage Using a DNA Movable-Type System

Zi-Yi Gong, Li-Fu Song, Guang-Sheng Pei, Yu-Fei Dong, Bing-Zhi Li, Ying-Jin Yuan

Journal Article

Clinical evaluation of recombinant human growth hormone injection in children with growth hormone deficiency

Ling HOU, Xiaoping LUO, Minlian DU, Huamei MA, Chunxiu GONG, Yuchuan LI, Shuixian SHEN, Zhuhui ZHAO, Li LIANG, Guanping DONG, Chaoying YAN, Hongwei DU

Journal Article

supplementation of thyroid hormone on treatment of idiopathic short-stature children during therapy with recombinant

Wei Wang, Shuqin Jiang, Zhirui Cui, Xiangyang Luo, Lingli Shi, Heli Zheng

Journal Article

The role of PARP1 in the DNA damage response and its application in tumor therapy

Zhifeng Wang, Fengli Wang, Tieshan Tang, Caixia Guo

Journal Article

Functional role of ATM in the cellular response to DNA damage

Ming LIU, Wenxiang HU

Journal Article

Generation and repair of AID-initiated DNA lesions in B lymphocytes

Zhangguo Chen,Jing H. Wang

Journal Article

Molecular simulation of the interaction mechanism between CodY protein and DNA in

Linchen Yuan, Hao Wu, Yue Zhao, Xiaoyu Qin, Yanni Li

Journal Article

Environmental pollution and DNA methylation: carcinogenesis, clinical significance, and practical applications

Yi Cao

Journal Article

Effects of DNA damage on oocyte meiotic maturation and early embryonic development

Shen YIN,Junyu MA,Wei SHEN

Journal Article

Single-Molecule Methods for Characterizing Different DNA Higher-Order Structures

Yonglin Liu, Tianyuan Bian, Yan Liu, Zhimin Li, Yufeng Pei, Jie Song

Journal Article